团队名称：

中英HUST-RRes基因工程与基因组学联合实验室

团队成员：

何光源 教授、杨广笑 教授、李引 副教授、常俊丽 副研究员、汪越胜 副教授、陈明洁 副教授、涂知明 副教授、王雅琼 博士后

团队简介：

团队于2000年组建，经过多年发展已建立起一支知识面宽、技术力量强、合作良好的科研团队，现有教师、博后、博士、硕士研究生35人。团队于2008年被科技部授予国际科技合作基地称号，与英国洛桑研究所、日本国立农业生物学研究所等单位建立了长期的科研合作关系。团队先后主持并完成国家转基因生物新品种培育重大专项、国家“863”计划、“973”计划子课题、科技部国际科技合作重点项目、国家自科基金等多项国家级课题。

实验室建筑面积约2,000平方米，拥有三间植物生长室、15平方米的低温实验室、150平方米的玻璃温室和300平方米的网室以及试验基地，拥有完备的基因分离、基因表达、作物遗传转化（小麦、水稻、玉米、高粱、烟草）和功能鉴定等研究平台和仪器设备，已形成植物基因工程、基因功能研究、作物品质研究和植物整合多组学四个科研平台。

团队的研究方向：

团队聚焦“植物功能基因组学与营养健康”这一核心方向，致力于理解植物感知内外部环境因子的基本过程和分子机制：包括感受外部干旱、盐害、极端温度以及内部的营养和信号分子等。与动物不同，植物因其固着生长特性，进化出了精密而复杂的机制来感受并适应各种不同的内外部环境因子。了解植物对内部营养状态的响应、与外部环境的互动以及在基因表达和代谢水平的调控至关重要，这将为应对气候变化、粮食与能源安全提供潜在的解决方案。团队改良植物对内部能量和代谢的调控以及对外部信号的响应与耐受，进而创制出更加适应气候变化、更加符合营养健康需求和粮食安全的智慧植物。

团队近年来的主要研究成果发表在Plant Biotechnology Journal、Biotechnology for Biofuels、Journal of Experimental Botany、Food Research International、Journal of Agricultural and Food Chemistry、Frontiers in Plant Science等国际知名植物学期刊，主要支撑了我校“农业科学”、“动物学与植物学”两个学科保持国际ESI学科排名前1%。

团队的代表性论文：

（1）小麦加工品质改良

Xiaoyan Li, Yin Li, Xiaofen Yu, Fusheng Sun, Guangxiao Yang, Guangyuan He. (2020) Genomics-enabled analysis of Puroindoline b2 genes identifies new alleles in wheat and related Triticeae species. International Journal of Molecular Sciences, 21, 1304.

Fusheng Sun, Xiaoxue Xie, Yufan Zhang, Jiangwei Duan, Mingyu Ma, Yaqiong Wang, Ding Qiu, Xinpei Lu, Guangxiao Yang, Guangyuan He. (2020) Effects of cold jet atmospheric pressure plasma on the structural characteristics and immunoreactivity of celiac-toxic peptides and wheat storage proteins. International Journal of Molecular Sciences, 21, 1012.

Yaqiong Wang, Miao Li, Yanbin Guan, Li Li, Fusheng Sun, Jiapeng Han, Junli Chang, Mingjie Chen, Guangxiao Yang, Yuesheng Wang, Guangyuan He. (2019) Effects of an additional cysteine residue of avenin-like b protein by site-directed mutagenesis on dough properties in wheat (Triticum aestivum L.). Journal of Agricultural and Food Chemistry, 67(31), 8559-8572.

Qiong Wang, Yin Li, Fusheng Sun, Xiaoyan Li, Pandi Wang, Guangxiao Yang, Guangyuan He. (2019) Expression of puroindoline a in durum wheat affects milling and pasting properties. Frontiers in Plant Science, 10, 482.

Fusheng Sun, Xiyan Liu, Qiuhui Wei, Jiannan Liu, Tianxiang Yang, Liyang Jia, Yuesheng Wang, Guangxiao Yang, Guangyuan He. (2017) Functional characterization of TaFUSCA3, a B3-superfamily transcription factor gene in the wheat. Frontiers in Plant Science, 8, 1133.

（2）小麦营养品质改良

Fusheng Sun, Jiannan Liu, Xiyan Liu, Yuesheng Wang, Kexiu Li, Junli Chang, Guangxiao Yang, Guangyuan He. (2017) Effect of the phytate and hydrogen peroxide chemical modifications on the physicochemical and functional properties of wheat starch. Food Research International, 100, 180-192.

Qiong Wang, Yin Li, Fusheng Sun, Xiaoyan Li, Pandi Wang, Jiutong Sun, Jian Zeng, Cheng Wang, Wei Hu, Junli Chang, Mingjie Chen, Yuesheng Wang, Kexiu Li, Guangxiao Yang, Guangyuan He. (2015) Tannins improve dough mixing properties through affecting physicochemical and structural properties of wheat gluten proteins. Food Research International, 69, 64-71.

Jian Zeng, Xiatian Wang, Yingjie Miao, Cheng Wang, Mingli Zang, Xi Chen, Miao Li, Xiaoyan Li, Qiong Wang, Kexiu Li, Junli Chang, Yuesheng Wang, Guangxiao Yang, Guangyuan He. (2015) Metabolic engineering of wheat provitamin A by simultaneously overexpressing CrtB and silencing carotenoid hydroxylase (TaHYD). Journal of Agricultural and Food Chemistry, 63(41), 9083-9092.

Cheng Wang, Jian Zeng, Yin Li, Wei Hu, Ling Chen, Yingjie Miao, Pengyi Deng, Cuihong Yuan, Cheng Ma, Xi Chen, Mingli Zang, Qiong Wang, Kexiu Li, Junli Chang, Yuesheng Wang, Guangxiao Yang, Guangyuan He. (2014) Enrichment of provitamin A content in wheat (Triticum aestivum L.) by introduction of the bacterial carotenoid biosynthetic genes CrtB and CrtI. Journal of Experimental Botany, 65(9), 2545-2556.

（3）综述类

Yin Li, Wenqin Wang, Chuang Ma, Ray Ming. (2021) Genomics-enabled crop genetics. Frontiers in Genetics, 12, 687160.

Min Tu, Yin Li. (2020) Toward the genetic basis and multiple QTL of kernel hardness in wheat. Plants. 9(12), 1631.

Xiaofen Yu, Qingchen Luo, Kaixun Huang, Guangxiao Yang, Guangyuan He. (2018) Prospecting for microelement function and biosafety assessment of transgenic cereal plants. Frontiers in Plant Science, 9, 326.

Jiapeng Han, Xiaofen Yu, Junli Chang, Guangxiao Yang, Guangyuan He. (2017) Overview of the wheat genetic transformation and breeding status in China. Methods in Molecular Biology, 1679, 37-60.

Yi He, Qiong Wang, Jian Zeng, Tao Sun, Guangxiao Yang, Guangyuan He. (2015) Current status and trends of wheat genetic transformation study in China. Journal of Integrative Agriculture, 14(3), 603457.

（4）植物耐受逆境的分子机制与遗传改良

Ding Qiu, Wei Hu, Yu Zhou, Jie Xiao, Rui Hu, Qiuhui Wei, Yang Zhang, Jialu Feng, Fusheng Sun, Jiutong Sun, Guangxiao Yang, Guangyuan He. (2021) TaASR1-D confers abiotic stress resistance by affecting ROS accumulation and ABA signalling in transgenic wheat. Plant Biotechnology Journal, 19(8), 1588-1601.

Jialu Feng, Lianzhe Wang, Yanan Wu, Qingchen Luo, Yang Zhang, Ding Qiu, Jiapeng Han, Peipei Su, Zhiyong Xiong, Junli Chang, Guangxiao Yang, Guangyuan He. (2019) TaSnRK2.9, a sucrose non-fermenting 1-related protein kinase gene, positively regulates plant response to drought and salt stress in transgenic tobacco. Frontiers in Plant Science, 9, 2003.

Chunlai Wu, Zehong Ding, Mingjie Chen, Guangxiao Yang, Weiwei Tie, Yan Yan, Jian Zeng, Guangyuan He, Wei Hu. (2019) Identification and functional prediction of lncRNAs in response to PEG and ABA treatment in cassava. Environmental and Experimental Botany, 166, 103809.

Qiuhui Wei, Qingchen Luo, Ruibin Wang, Fan Zhang, Yuan He, Yang Zhang, Ding Qiu, Kexiu Li, Junli Chang, Guangxiao Yang, Guangyuan He. (2017) A Wheat R2R3-type MYB transcription factor TaODORANT1 positively regulates drought and salt stress responses in transgenic tobacco plants. Frontiers in Plant Science, 8, 1374.

（5）植物代谢调控

Min Tu, Yin Li. (2020) Profiling alternative 3’ untranslated regions in Sorghum using RNA-seq data. Frontiers in Genetics, 11, 556749.

Yin Li, Min Tu, Yaping Feng, Wenqin Wang, Joachim Messing. (2019) Common metabolic networks contribute to carbon sink strength of sorghum internodes: implications for bioenergy improvement. Biotechnology for Biofuels, 12, 274.

Yin Li, Wenqin Wang, Yaping Feng, Min Tu, Peter E Wittich, Nicholas J Bate, Joachim Messing. (2019) Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in different sorghum genotypes. Plant Biotechnology Journal, 17(2), 472-487

Yin Li, Rushabh Mehta, Joachim Messing. (2018) A new high-throughput assay for determining soluble sugar in sorghum internode-extracted juice. Planta, 248(4), 785-793.