华中科技大学生命科学与技术学院
2019暑期欧洲贝时璋菁英班研学夏令营日志
Day 10: 来到德国哥廷根
今天是到德国的第三天，开始x-club课程的第一天。
早上乘坐公交车到x-club，虽然不懂德语，但还是可以直接按照已给的时间表来得知到站与否，时间表上的8：56到达的公交，准时到达了四色组成的实验楼，上到绿色的生物实验楼层开始了自己的课程。
实验千万条，安全第一条，名字老师上来就介绍了生物实验室的四个等级，简单来说一级无害，二级要避免咬伤，三级要避免体液传播，四级要全方位防卫，比如埃博拉病毒。之后签字表示我们知道实验室等级，并会遵守实验室规则。尤其是防火通道在平时不能通过，有室内和室外两个，就感觉德国对安全的重视，自己也因他们的重视而感到安全。
进而开始了我们的课程，从DNA的定义，到质粒本身作为基因克隆工具的优势，再到今天的实验介绍：提取质粒，限制酶酶切，电泳检验，转化。
在正式实验之前，先讲解了仪器的使用注意事项，比如EP管需要用镊子取出、取出枪头之后要及时关闭盒盖，避免污染。感觉实验操作非常严谨，算是体现了德国人的认真地态度，自己还是有不少要改进的地方。
再进行酶切和胶块的制作，其中温度还是有专门的温度计来记录，相对于自己之前用手感受，就显得非常的准确了。
趁着酶切的一小时，大家都去吃食堂的午饭了，有水果，蔬菜，蔬菜和一杯饮料，算是非常的丰富了！在回去的路上，大家都进入到游戏时间，变成大孩子，一起跷跷板，荡秋千，滑滑梯，可以说非常有趣了！
完成感受态细胞制作后大家来到哥廷根的市中心，在学长兼导游的带领下，我们参观了市中心。我们来到牧鹅女雕像所在地，每年都会有博士毕业的学生在老师和家人的陪伴下来亲吻雕像，这已成学校的传统。后来到了威廉四世送的礼堂，导游详细讲解了屋顶的雕像，主礼堂和学生监狱，我们学到了不少德国历史和科学史。
最后大家下了公交，走在德国的夕阳之下，远方是有着金边的暗红色木屋，看到自己旁边嬉笑的同伴，感受着生活的充实与平静，默念自己也要继续加油呀，便投入了欢笑的海洋之中。Guten Abend allerseits!
Today is the third day in Germany and the first day of x-club course.
In the morning, we took the bus to x-club. Although we didn't know German, we could know whether the bus arrived or not according to the given timetable. The bus arrived at 8:56 on the timetable and arrived at the four-color laboratory building on time.
Thousands of experiments, the first safety. Professor Maram Bader introduced the four levels of the biological laboratory , simply meaning harmless first, second to avoid bites, third to avoid the transmission of body fluids, four to all-round defense.We sign to know the laboratory level and will abide by the laboratory rules.
Then we began our course, from the definition of DNA, to the advantages of plasmids themselves as gene cloning tools, to today's experiment introduction: plasmids extraction, restriction enzyme digestion, electrophoresis test, transformation.
Before the formal experiment, the precautions for the use of the instrument were explained. For example, the EP tube should be taken out with tweezers, and the lid of the box should be closed timely after the gun head was taken out to avoid contamination.We felt that the operation of the experiment is very rigorous, which reflects the German people's serious attitude.
After that, enzyme cutting and glue making were carried out, and the temperature was recorded by a special thermometer, which was very accurate compared with the feeling by hand before.
After the production of competent cells, we went to the city center of gottingen. Under the guide of the Mentor of HUST, we visited the city center. We came to the place where the goose-girl statue is. Every year, students with doctorate degree will be accompanied by teachers and family members to kiss the statue, which has become a school tradition. Later, we went to the auditorium where William IV donated. We learned a lot about German history and the history of science，such as the roof, the main auditorium and the students’ prison.
Finally, we got off the bus, walking in the Germans’ sunset.The dark red wooden house is in the distance. Listen to companions’ laughing, we felt the life of enrichment and calmness.Fight tomorrow!Guten Abend allerseits!
