题目1 ：Neuronal signaling from structural biology perspective

主讲人：Zhang Mingjie Chair Professor of Biochemistry

Department of Biochemistry, Hong Kong University of Science and Technology

题目2：Visualizing functions from protein structure in neuronal development

主讲人：Wei Zhiyi Ph.D.

Department of Biochemistry, Hong Kong University of Science and Technology

题目3：To Move or Not to Move: who gives instructions to the cellular transportation vehicles like myosin VI

主讲人：Yu Cong Ph.D.

Department of Biochemistry, Hong Kong University of Science and Technology

题目4：Structural Characterization of Usher Syndrome Interactome Assembly

主讲人：Pan Lifeng Ph.D.

Department of Biochemistry, Hong Kong University of Science and Technology

时间：2009年12月24日上午9:00

地点：东十一楼二楼会议室

 

- B.Sc. in Chemistry                                                                       1984-1988

 Fudan University (Shanghai, P.R. China)

-Ph.D. in Biochemistry                                                                 1989-1993

 University of Calgary (Calgary, Canada)

-Postdoctoral Fellow                                                                      1994-1995

 Ontario Cancer Institute, University of Toronto (Canada)

- Graduate student (Ph.D.), Department of Biological Sciences,                      1989-1993

 University of Calgary (Supervisor Dr. H. J. Vogel)

- Postdoctoral Fellow, Department of Biological Sciences,                          Jan. 1994-

- Postdoctoral Fellow, Division of Structural and Molecular Biology             Jun. 1994-

- Assistant Professor, Department of Biochemistry                                  1995-1999

- Associate Professor, Department of Biochemistry                                 2000-2004

- Professor, Department of Biochemistry                                             2004-2008

- Chair Professor, Department of Biochemistry                              2008-date

- Regular reviewer for academic journals: Mol Cell, Nat. Struct. & Mol. Biol., PNAS, J. Biol. Chem., J Cell Sci., JACS, Biochemistry, Mol. Cell. Biol., J. Mol. Biol., Structure, PLoS One, Proteins, J. Med. Chem., Eur. J. Biochem., FEBS Lett., Neurolsignals, Magn. Reson. Chem.etc.

-Reviewer for grant proposals: The Wellcome Trust (Joint Infrastructure Fund and Project Grants), The Human Frontier Science Program Research Grant, National Science Foundation (USA), Medical Research Council of UK, National Science & Engineering Research Council of Canada, The Biotechnology and Biological Sciences Research Council of UK, the Council for Chemical Sciences of the Netherlands, The Northwest Health Foundation of USA, The Grant Research Council of Hong Kong

- Panel member, Biology and Medicine Panel, The Research Grant Council of Hong Kong

- Fellowship Committee Member, The Human Frontier Science Program

- Member of the Academic Committee, Beijing NMR Center, China

- Member of the Academic Committee, HKUST-Chinese Academy of Science Life Science Joint Laboratory

- Director, HKUST-Chinese Academy of Science Life Science Joint Laboratory

- Director, HKUST-Peking University Shenzhen Biomedical Research Institute

- Deputy Director, HKUST-Peking University Shenzhen Biomedical Center

- Guest Lecturer, Bio2000 Lecture Series, Chinese Academy of Science

- Adviser, Overseas Scientific Evaluation Committee, Chinese Academy of Science

- International Advisor, The 1st , 2nd and 3rd Asia Pacific NMR Symposiums

- Board Member, Asia Pacific NMR Society

- Member of the Academic Committee, State Key Laboratory of Magnetic Resonance and Molecular and Atomic Physics, Chinese Academy of Science (2005-2009)

1. Zhang, M. and Vogel, H.J. (1993) "Protein Engineering and NMR Studies of Lysine Residues in Mammalian Calmodulin". Bull. Magn. Reson. 15, 95-97.

2. Zhang, M. and Vogel, H.J. (1993) "Structure Determination of the Calmodulin Binding Domain of Caldesmon" In"Peptides: Chemistry, Structure and Biology" (Eds. R.S. Hodges, J.A. Smith), Escom. Science Publ., Leiden, The Netherland, pp963-965.

3. Zhang, M. and Vogel, H.J. (1993) "Determination of the Side Chain pKa Values of the Lysine Residues in Calmodulin". J. Biol. Chem. 268, 22420-22428.

4. Zhang, M., Yuan, T. and Vogel, H.J. (1993) "A Peptide Analog of the Calmodulin-binding Domain of Myosin Light Chain Kinase Adopts an a-Helical Structure in Aqueous Trifluoroethanol". Prot. Sci. 2, 1931-1937.

5. Zhang, M. and Vogel, H.J. (1994) "Characterization of the Calmodulin-binding Domain of Rat Cerebellar Nitric Oxide Synthase". J. Biol. Chem. 269, 981-985.

6. Zhang, M., Huque, E. and Vogel, H.J. (1994) "Characterization of Trimethyllysine-115 in Calmodulin by Nitrogen-14 and Carbon-13 NMR" J. Biol. Chem. 269, 5099-5105.

7. Zhang, M. and Vogel, H.J. (1994) "The Calmodulin-binding Domain of Caldesmon Binds to Calmodulin in an a-Helical Conformation." Biochemistry  33 ,1163-1171.

8. Zhang, M. and Vogel, H.J. (1994) "Two Dimensional NMR Studies of Selenomethionyl Calmodulin" J. Mol. Biol. 239, 545-554.

9. Zhang, M., Li, M., Wang, J. and Vogel, H.J. (1994) “The Effect of Met®Leu Mutations on Calmodulin's Ability to Activate Cyclic Nucleotide Phosphodiesterase” J.Biol.Chem. 269, 15546-15552.

10. Zhang, M., Vogel, H.J. and Zwiers, H. (1994) “Nuclear Magnetic Resonance Studies of the Structure of B50/Neuromodulin and its interaction with Calmodulin." Biochem. Cell Biol. 72, 109-116.

11. Zhang, M., Thulin, E. and Vogel, H. J.(1994) “Determination of Lysine pKa’s in Calbindin D9k” J. Prot. Chem. 13, 527-535.

12. Zhang, M., Fabian, H., Mantsch, H., and Vogel, H. J. (1994) “Isotope-Filtered Fourier Transformed Infrared Spectroscopy Studies of the Interaction of Calmodulin with its Target Peptides”. Biochemistry 33, 10883-10888.

13. Siivari, K., Zhang, M., Palmer, A. G., and Vogel, H. J. (1995) "NMR Studies of the Methionine Methyl Groups in Calmodulin". FEBS Lett.  366, 104-108.

14. Vogel, H.J. and Zhang, M. (1995) “Protein Engineering and NMR Studies of the Methionine Residues of Calmodulin”. Mol. Cell. Biochem. 149/150, 3-15.

15. Zhang, M., Tanaka, T. and Ikura, M. (1995) "Calcium-induced Conformational Transition Revealed by the Solution Structure of Apo Calmodulin". Nature Struct. Biol. 2, 758-767.

16. Zhang, M., Yuan, T., Aramini, J. M., and Vogel, H. J. (1995) "Interaction of Calmodulin with it Binding Domain of Rat Cerebellar Nitric Oxide Synthase: A Multinuclear NMR Study". J. Biol. Chem. 270, 20901-20907.

17. Zhang, M., Tanaka, T., Farrow, N.A., Kay, L.E. and Ikura, M. (1995) "Multidimensional NMR Studies of the Structure and Dynamics of Calcium Free Calmodulin". Prot. Eng. 8, S30.

18. Aramini, J. M., Hiraoki, T., Yazawa, M., Yuan, T., Zhang, M., and Vogel, H. J. (1996) "Lead-207 NMR: A Novel Probe for the Study of Calcium-Binding Proteins". J. Biol. Inorgan. Chem. 1, 39-48.

19. Martin, S. R., Bayley, P. M., Brown, S. E., Porumb, T., Zhang, M. and Ikura, M. (1996) "Spectroscopic Characterization of a High-Affinity Calmodulin-Target Peptide Hybrid Molecule" Biochemistry  35, 3508-3517.

20. Ohki, S., Ikura, M., and Zhang, M. (1997) "Identification of the Mg2+-binding Sites and Role of Mg2+ on Target Recognition by Calmodulin". Biochemistry   36, 4309-4316.

21. Tang, D., Chun, A.C.S., Zhang, M. and Wang, J.H. (1997) "Cdk5 Activation and Binding Sequences of Neuronal Specific Cdk5 Activator------Evidence of the existence of cyclin fold in Nck5a". J. Biol. Chem. 272, 12318-12327.

22. Zhang, M., and Vogel, H. J. (1997) "Interaction of a Partial Calmodulin-Binding Domain of Caldesmon with Calmodulin" Prot. Pept Lett. 4, 291-297.

23. Ohki, S., Zhang, Q., Li, M. and Zhang, M. (1997) “NMR Secondary and Biochemical Studies of an Associated Protein Inhibitor of Neuronal Nitric Oxide Synthase, PIN” Symposium on Frontiers of Chemistry 49-50.

24. Zhang, M. and Yuan, T. (1998) "Molecular Mechanisms of Calmodulin's Functional Versatility" Biochem. Cell Biol. 76, 313-323. (invited).

25. Tochio, H., Ohki, S., Zhang, Q., Li, M., and Zhang, M. "Solution Structure of a Protein Inhibitor of Neuronal Nitric Oxide Synthase" (1998) Nature Struct. Biol. 5, 965-969.

26. Fan, J.-S., Zhang, Q., Li, M., Tochio, H., Yamazaki, T., Shimizu, M., and Zhang, M.   "Protein Inhibitor of Neuronal Nitric Oxide Synthase (PIN) Binds to a 17 Amino Acid Residues Fragment of the Enzyme" (1998) J. Biol. Chem. 273, 33472-33481.

27. Fan, J-S., Cheng, H.-C., and Zhang, M. "A Peptide Corresponding to the Residues Asp177 to Asn208 of Human Cyclin A Forms an a-helix" (1998) Biochem. Biophys. Res. Commun. 253, 621-627.

28. Chin, C.-T., Ohki, S., Tang, D., Cheng, H.-C., Wang, J. H., and Zhang, M. (1999)   "Identification and Structural Characterization of a Cdk Inhibitory Peptide Derived From Neuronal Specific Cdk5 Activator". J. Biol. Chem.  274, 7120-7127.

29. Tochio, H., Zhang, Q., Mandal, P., Li., M., and Zhang, M. "Solution Structure of the Extended Neuronal Nitric Oxide Synthase PDZ Domain Complexed with an Associated Peptide" (1999) Nature Struct. Biol.  6, 417-421.

30. Lo, K. W.-H., Zhang, Q., Li, M., and Zhang, M. (1999) "Apoptosis-linked Gene ALG-2 Is a New Member of Calpain Small Subunit Subfamily Ca²⁺-binding protein" Biochemistry 38, 7498-7508.

31. Tochio, H., Hung, F., Li, M., Bredt, D. S., and Zhang, M. (2000) “Solution Structure and Backbone Dynamics of the Second PDZ Domain of Postsynaptic Density-95”. J. Mol. Biol. 295, 225-237.

32. Wang, P., Zhang, Q., Tochio, H., Fan, J.-S., and Zhang, M. (2000) “Formation of a Native Like b-Hairpin Finger Structure of a Peptide from the Extended PDZ Domain of Neuronal Nitric Oxide Synthase in Aqueous Solution.” Eur. J. Biochem. 267, 3116-3122,

33. Wang, X., Ching, Y.-P, Lam, W.-H., Qi, Z., Zhang, M., and Wang, J. H. (2000) “Identification of a Common Protein Association Region in the Neuronal Cdk5 Activator” J. Biol. Chem. 275, 31763-31769,

34. Tochio, T., Mok, Y-K, Zhang, Q., Kan, H.-M., Bredt, D. S., and Zhang, M. (2000) “Formation of nNOS/PSD-95 PDZ Dimer Requires a Preformed b-Finger Structure from the nNOS PDZ Domain” J. Mol. Biol. 303, 359-370.

35. Fan, J.-S, Zhang, Q., Tochio, H. Li, M., and Zhang, M. (2001) “Structural Basis of Diverse Sequence-dependent Target Recognition by the 8 kDa Dynein light Chain” J. Mol. Biol. 306, 97-108.

36. Lo, K. W.-H., Naisbitt, S., Fan, J.-S, Sheng, M. and Zhang, M. (2001) "The 8 kDa Dynein Light Chain Binds to its Targets via a Conserved (K/R)XTQT Motif" J. Biol. Chem. 276, 14059-14066.

37. Mok, Y.-K., Lo, K. W.-H., and Zhang, M. (2001) "Structure of Tctex-1 and its Interaction with Cytoplasmic Dynein Intermediate Chain" J. Biol. Chem. 276, 14067-14074.

38. Tochio, T., Tsui, M. M. K., Banfield, D. K., and Zhang, M. (2001) "An Auto-Inhibitory Mechanism for Non-Syntaxin SNARE Proteins Revealed by the Structure of Ykt6p" Science 293, 698-702.

39. Zhang, Q., Fan, J.-S., and Zhang, M. (2001) “Inter-domain Chaperoning Between PSD-95, Dlg, and Zo-1 (PDZ) Domains of Glutamate Receptor Interacting Proteins” J. Biol. Chem. 276, 43216-43220

40. Wu F, Zhang M, and Gong W. (2001) “Crystallization and preliminary crystallographic studies of an apoptosis-linked calcium-binding protein ALG-2” Acta Crystallogr D Biol Crystallogr 57, 1162-1163.

41. Fan, J.-S, Zhang, Q., Tochio, H., and Zhang, M. (2002) “Backbone Dynamics of the 8 kDa Dynein Light Chain Dimer Reveals Molecular Basis of Proteins Functional Diversity” J. Biol. Mol. NMR 23, 103-114.

42. Galigniana, M.D., Harrell, J. M., Murphy, P. J., Chinkers, M., Radanyi, C., Renoir, J.-M., Zhang, M., and Pratt, W. B. (2002) “Binding of hsp90-associated Immunophilins to Cytoplasmic Dynein: Direct Binding and in vivo Evidence that the Peptidylpropyl Isomerase Domains is a Dynein Interaction Domain” Biochemistry 41, 13602-13610.

43. Feng, W., Fan, J.-S., Jian, M., Shi, Y.-W., and Zhang, M. (2002) “PDZ7 of Glutamic Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area” J. Biol. Chem. 277, 41140-41146.

44. Fan, J.-S., and Zhang, M. (2002) “Signaling Complex Organization by PDZ Domain Proteins” NeuroSignals 11, 315-321. (invited review)

45. Long, J., Tochio, H., Wang, P., Fan, J.-S, Sala, C., Niethammer, M., Sheng, M. and Zhang, M. (2003) " Supramodular Structure and Synergistic Target Binding of the N-terminal Tandem PDZ Domains of PSD-95" J. Mol. Biol. 327, 203-214.

46. Zhang, M., and Wang, W. (2003) “Organization of Signaling Complexes by PDZ-Domain Scaffold Proteins” Acc. Chem. Res. 36, 530-538. (invited review).

47. Fang, M., Tao, Y. X., He, F., Zhang, M., Levine, C. F., Mao, P., Tao, F., Chou, C. L., Sadegh-Nasseri, S., Johns, R. A. (2003) “Synaptic PDZ Domain-mediated Protein Interactions Are Disrupted by Inhalational Anesthetics” J. Biol. Chem. 278, 36669-36675.

48. Tang, W., Sun, X., Fang, J.-S., Zhang, M., and Sucher, N. J. (2004) “Flavonoids from Radix Scutellariae as potential stroke therapeutic agents by targeting the second postsynaptic density 95 (PSD-95)/disc large/zonula occludens-1(PDZ) domain of PSD-95.” Phytomed. 11, 277-284.

49. Feng, W., Shi, Y., Li, M. and Zhang, M. (2003) “Tandem PDZ repeats in glutamate receptor interacting proteins have a novel mode of PDZ domain-mediated target binding.” Nat. Struct. Biol. 10, 972-978.

50. Wang, W, Lo, K. W.-H., Kan, H.-M., Fan, J.-S., and Zhang, M. (2003) “Structure of the monomeric 8-kDa dynein light chain and mechanism of the domain swapped dimer assembly” J. Biol. Chem. 278, 41491-41499.

51. Zheng, Q., Yang, W.M., Yu, W.H., Cai, B., Teng, X.C., Xie, Y., Sun, H.Z., Zhang, M., Huang, Z.X. (2003) “The effect of the EAAEAE insert on the property of human metallothionein-3” Protein Eng. 16, 865-870.

52. Feng, W., Long, J., Fan, J.-S., Tetsuya, S., and Zhang, M. (2004) “Hetero-tetrameric L27 (Lin-2, Lin-7) domain complexes as organization platforms for supra-molecular assemblies” Nat. Struct. & Mol. Biol. 11, 475-480.

53. Xu, X.-F., Tan, Y.-W., Lam, L., Hackett, J., Zhang, M., and Mok, Y.-K. (2004) “NMR structure of a type IVb Pilin from Salmonella typhi and its assembly into Pilus”279, 31599-31605. J. Biol. Chem.

54. Romorini, S., Piccoli, G., Jiang, M., Grossano, P., Tonna, N., Passafaro, M., Zhang, M. and Sala, C. (2004) "A functional role of PSD-95/GKAP complex in regulating Shank assembly and stability to synapses" J. Neurosci. 24, 9391-9404.

55. Cheung, P.-Y., Zhang, Y., Long, J., Lin, S., Zhang, M., Jiang, Y. and Wu, Z. (2004) “p150^Glued, dynein and microtubules are specifically required for activation of MKK3/6 and p38 MAPKs” J. Biol. Chem. 279, 45308 – 45311.

56. Lo, K. W.-H., Kan, H.-M., Chan, L.-N., Xu, W.-G., Wang, K.-P., Wu, Z., Sheng, M., and Zhang, M. (2005) “The 8 kDa dynein light chain binds to 53BP1 and mediates DNA damage-induced p53 nuclear accumulation.” J. Biol. Chem. 280, 8172-8179.

57. Aouacheria, A., Navratil, V., Wen, W., Jiang, M., Mouchiroud, D., Gautier, C., Gouy, M., and Zhang, M. (2005) “In silico whole-genome scanning and characterization of tumour-associated nonsynonymous SNPs using EST data” Oncogene 24, 6133-6142.

58. Feng, W., Long, J., and Zhang, M. (2005) “A unified assembly mode revealed by the structures of tetrameric L27 domain complexes formed by mLin-2/mLin-7 and Patj/Pals1 scaffold proteins”. Proc. Natl. Acad. Sci. USA 102, 6861-6866.

59.      Long, J.-F., Feng, W., Wang, R., Chan, L.-N., Ip, F.C.F., Xia, J., Ip, N., and Zhang, M. (2005) “Auto-inhibition of X11s/Mints Scaffold Proteins Revealed by the Closed Conformation of the PDZ Tandem” Nat. Struct. & Mol. Biol. 12, 722-728.

60.      Yan, J., Wen, W., Xu, W., Long, J.-F., Adams, M. E., Froehner, S.C,a1-Syntrophin” EMBO J. 24, 3985-3995.and Zhang, M. (2005) “Structure of the Split PH domain and Distinct Lipid Binding Properties of the PH-PDZ Supramodule of

61.      Yang, Z., Xu, K., Wang, L., Gu, H., Wei, H., Zhang, M, and Xu, B. (2005) “Self-assembly of Small Molecules Affords Multifunctional Supramolecular Hydrogels for Topically Treating Simulated Uranium Wounds” Chem. Comm. 35, 4414-4416.

62.      Ding, Z. C., Teng, X.C., Cai, B., Wang, H., Zheng, Q., Wang, Y., Zhou, G. M., Zhang, M., Wu, H. M., Sun, H.Z., and Huang, Z.X. Biochem Biophys Res Commun. 349, 674-682. (2006) “Mutation at Glu23 eliminates the neuron growth inhibitory activity of human metallothionein-3”

63.      Wan, J., Zhang, W., Wu, L., Bai, T., Zhang, M., Lo, K.-W., Chui, Y., Cui, Y., Tao, Q., Yamamoto, M., Akira, S., Wu, Z. (2006) “BS69 Is a Specific Adaptor in the Latent Membrane Protein 1-meadiated c-Jun N-terminal Kinase Pathway” Mol. Cell. Biol. 26, 448-456.

64.      Cai, B., Zheng, Q., Teng, X.C., Chen, D., Wang, Y., Wang, K.Q., Zhou, G.M., Xie, Y., Zhang, M., Sun, H.Z., and Huang, Z.X. (2006) “The role of Thr5 in human neuron growth inhibitory factor” J Biol Inorg Chem. 11, 476-682.

65.      Jin, W., Ge, W., Xu, J., Cao, M., Peng, L., Yung, W., Liao, D., Duan, S., Zhang, M., and Xia, J. (2006) "Lipid Binding Regulates PICK1s Synaptic Targeting, AMPA Receptor Trafficking and Synaptic Plasticity" J. Neurosci. 26, 2380-2390.

66.      Wen, W., Yan, J., and Zhang, M. (2006) “Structural characterization of the split PH domain in phospholipase C-g1 and its interaction with TRPC3” J. Biol. Chem. 281, 12060-12068.

67.      Wen, W., Chen, L., Wu, H., Sun, X., Zhang, M. and Banfield, D.K. (2006) “Identification of the yeast R-SNARE Nyv1p as a novel longin domain-containing protein” Mol. Biol. Cell 17, 4282-4299

68.      Wen, W., Wang, W., and Zhang, M. (2006) “Targeting PDZ Domain Proteins for Treating NMDA Receptor-mediated Excitotoxcicity” Curr. Top. Med. Chem. 6, 711-721.

69.      Feng, W., Wu, H., Chan, L.-N, and Zhang, M. (2007) “The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and apical membrane localization” EMBO J. 26,2786-2796.

71.      Feng, W., Huang, S., Wu, H., and Zhang, M. (2007) “Molecular basis of Bcl-xL’s target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of Beclin-1“ J. Mol. Biol. 372, 223-235.

72.      Olsen, O., Funke, L., Long, J.-F., Fukata, M., Kazuta, T., Trinidad, J. C., Moore, K. A., Misawa, H., Welling, P. A., Burlingame, A. L., Zhang, M., and Bredt, D. S. (2007) ”Renal Agenesis, Cysts and Fibrosis Associated with Improper Localization of the CRB and DLG Polarity Complexes in MALS-3 Knockout Mice” J. Cell Biol. 179, 151-164.

73.      Wu, H, Feng, W., Chen, J., Chan, L.-N., Huang, S., and Zhang, M. (2007) “PDZ Domains of Par-3 as Potential Phosphoinositide Signalling Integrators” Mol Cell 28, 886-898.

74.      Pan, L. F., Wu, H., Shen, C., Shi, Y., Jin, W., Xia, J., and Zhang, M. (2007) “Clustering and synaptic targeting of PICK1 requires direct interaction between the PDZ domain and lipid membranes” EMBO J 26, 4576-4587.

75.      Zhang, M. (2007) “Scaffold proteins as dynamic switches” Nat. Chem. Biol. 3, 756-757.

76.      Long, J.-F., Wei, Z., Feng, W., Yu, C., Zhao, Y., and Zhang, M. (2008) “Supramodular Nature of GRIP1 Scaffold Revealed by the Crystal Structure of Its PDZ12 Tandem in Complex with the Carboxyl Tail of Fras1” J. Mol. Biol. 375, 1457-1468.

77.      Song, C., Wen, W., Rayala, S. K., Singh, R. R., Chen, M., Ma, J., Talukder, A. H., Zhang, M.^* and Kumar, R.* (2008) “Serine 88 Phosphorylation of the Dynein Light Chain 1 is a Molecular Switch for its Dimerization Status and Functions” J. Biol. Chem. 283, 4404-4413 (*corresponding authors).

78.      Yan, J., Wen, W., Chan L.-N., and Zhang, M. (2008) “Split PH domain-mediated cytoplasmic-nuclear localization of PI3-kinase enhancer GTPase” J. Mol. Biol. 378, 425-435.

79.      Wan, J., Cheung, A. Y., Fu, W.-Y., Wu, C., Zhang, M., Mobley, W. C., Cheung, Z. H., and Ip, N. Y. (2008) “Inhibition of endophilin B1 modulates TrkA intracellular trafficking and NGF-induced neurite outgrowth” J. Neurosci. 28, 9002-9012.

80.      Feng, W., Wu, H, Chan, L.-N., and Zhang, M. (2008) ”Par-3-mediated Junctional Localization of the Lipid Phosphatase PTEN is Required for Cell Polarity Establishment”  J. Biol. Chem. 283, 23440-23449.

81.      Chen, J., Pan, L. F., Wei, Z., Zhao, Y. and Zhang, M. (2008) “Domain Swapped Dimerization of ZO-1 PDZ2 Generates Specific and Regulatory Connexin43 Binding Sites” EMBO J 27, 2113-2123.

82.      Wen, W., Liu, W., Yan, J., and Zhang, M. (2008) “Structure basis and unconventional lipid membrane binding properties of the PH-C1 tandem of Rho-kinases” J. Biol. Chem. 283, 26263-26273.

83.      Feng, W., Zhang, M. (2009) “Organization and Dynamics of PDZ Domain-Related Supramodules in the Postsynaptic Density” Nat. Rev. Neurosci. 10, 87-99.

84.      Wang, W., Weng, J., Zhang, X., Liu, M., and Zhang, M. (2009) “Creating conformational entropy by increasing inter-domain mobility in ligand binding regulation: a revisit to N-terminal tandem PDZ domains of PSD-95”  J. Am. Chem. Soc. 131, 787-796.

85.      Wang, R., Wei, Z., Jin, H., Wu, H., Yu, C., Wen, W., Chan, L.-N., Wen, Z., and Zhang, M. (2009) “Auto-inhibition of UNC5b Revealed by the Cytoplasmic Domain Structure of the Netrin Receptor” Mol Cell  33, 692-703.

86.      Pan L. F., Yan, J., Wu, L., and Zhang, M. (2009) “Assembling Stable Hair Cell Tip Link Complex via Multi-dentate Interactions between Harmonin and Cadherin23” PNAS 106, 5575-5580.

87.      Yu, C., Feng, W., Wei, Z., Miyanoiri, Y., Wen, W., Zhao, Y., and Zhang, M. (2009) “Myosin VI undergoes cargo-mediated dimerization” Cell 138, 537-548.

88.      Gianni, S., Brunori, M., Jemth, P., Oliveberg, M., and Zhang, M. (2009) “Distinguishing between smooth and rough free energy barriers in protein folding” Biochemistry 48, 11825-11830.

89.      Wen, W., Yu, J., Pan, L., Weng, J., Wang, W., Ong, Y. S., Hoai, T., Hong, W. and Zhang, M. (2009) “Lipid-induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6”. Mol Cell (in press).

90.      Yan, J., Pan L. F., Chen, X., Wu, L., and Zhang, M. (2009) “The Structure of the Harmonin/Sans Complex Reveals an Unexpected Interaction Mode of the Two Usher Syndrome Proteins”. PNAS (in press)

91.      Wei, Z., Zheng, S., Yu, C., and Zhang, M. (2009)  “The Structure of the Liprin-alpha/CASK Complex Reveals a Mammalian Specific Presynaptic Protein Organization Mode” Neuron (under revision).

92.      Wen, W., Wang, R., Kam, C., Xia, J., and Zhang, M. (2009) “Discovery and structural basis of the ZU5 domain as a target binding module regulating cellular localization of ZO-1” (under revision).

93.      Long, J.-F., Chan, L,-N., Yan, J., Jun, X., and Zhang, M. (2009) ”A polybasic lipid membrane binding motif mediates clustering of GRIP1“ (submitted).

 

1.Wei Z, Zheng S, Yu C, Zhang M. The Structure of the liprin-α/CASK complex reveals a mammalian specific presynaptic protein organization mode. Neuron. In revising.

2.Wang R, Wei Z(co-first author), Jin H, Wu H, Yu C, Wen W, Chan LN, Wen Z, Zhang M. Autoinhibition of UNC5b revealed by the cytoplasmic domain structure of the receptor. Mol Cell. 27: 692-703 (2009)

3.Long J, Wei Z(co-first author), Feng W, Yu C, Zhao Y, Zhang M. Supramodular nature of GRIP1 revealed by the structure of its PDZ12 tandem in complex with the carboxyl tail of Fras1. J Mol Biol. 375:1457-68 (2008)

4.Wei Z, Xue Y, Xu H, Gong W. Crystal structure of the C-terminal Domain of S.cerevisiae eIF5. J Mol Biol. 359: 1-9 (2006)

5.Wei Z, Zhang P, Zhou Z, Cheng Z, Wan M, Gong W. Crystal structure of human eIF3k, the first structure of eIF3 subunits. J Biol Chem. 279: 34983-90 (2004)

6.Liu L, Wei Z(co-first author), Wang Y, Wan M, Cheng Z, Gong W. Crystal structure of human coactosin-like protein. J Mol Biol. 344: 317-23 (2004)

7.Yu C, Feng W, Wei Z, Miyanoiri Y, Zhao Y, Zhang M. Structural insights into the cargo-binding mediated dimerization of Myosin VI Cargo binding-regulated conversion of Myosin VI from a nonprocessive monomer into a processive dimer. Cell.138: 537-48 (2009)

8.Gong W, Hao B, Wei Z, Ferguson D, Tallant T, Krzycki J, Chan M. Structure of the alpha2epsilon2 Ni-dependent CO dehydrogenase component of the Methanosarcina barkeri acetyl-CoA decarbonylase/synthase complex. Proc Natl Acad Sci USA. 105:9558-63 (2008)

9.Zhang M, Wei Z, Chang S, Teng M, Gong W. Crystal structure of a papain-fold protein without the catalytic residue: A novel member in the cysteine proteinase family. J Mol Biol. 358: 97-105 (2006)

10.              Li J, Wei Z, Zheng M, Gu X, Deng Y, Qiu R, Chen F, Ji C, Gong W, Xie Y, Mao Y. Crystal structure of human guanosine monophosphate reductase 2 (GMPR2) in complex with GMP. J Mol Biol. 355: 980-8 (2006)

11.              Wang Y, Wei Z, Bian Q, Cheng Z, Wan M, Liu L, Gong W. Crystal structure of human bisphosphoglycerate mutase. J Biol Chem. 279: 39132-38 (2004)

 

1.          Yu, C.*, Feng, W.*, Wei, Z., Miyanoiri, Y., Wen, W., Zhao, Y., and Zhang, M. (2009). Myosin VI Undergoes Cargo-Mediated Dimerization. Cell 138, 537-548.

2.          Wei, Z. *, Zheng, S. *, Yu, C.^# and Zhang, M.^# (2009). The Structure of the Liprin-a/CASK Complex Reveals a Distinct Presynaptic Protein Complex Organization Mode in Mammals. Submitted to Neuron

3.          Feng, W.*, Yu, C.*, Wu, J., Pan, L., and Zhang, M. (2009). Cargo-induced lever arm expansion allows myosin VI to walk with large and variable step sizes. Submitted to PNAS.

4.          Long, J.*, Wei, Z.*, Feng, W., Yu, C., Zhao, Y. X., and Zhang, M. (2008). Supramodular nature of GRIP1 revealed by the structure of its PDZ12 tandem in complex with the carboxyl tail of Fras1. J Mol Biol 375, 1457-1468.

5.          Wang, R.*, Wei, Z.*, Jin, H., Wu, H., Yu, C., Wen, W., Chan, L. N., Wen, Z., and Zhang, M. (2009). Autoinhibition of UNC5b revealed by the cytoplasmic domain structure of the receptor. Mol Cell 33, 692-703.

 

-B.Sc., Department of Chemistry, Fudan University

 (Shanghai, P.R.China)                 9. 2001 - 7. 2005

-Ph.D. candidate, Department of Biochemistry, The Hong Kong University

of Science and technology (Hong Kong, P.R.China)                           9. 2005 - present

1.Pan, L.*, Wu, H.*, Shen, C.*, Shi, Y., Jin, W., Xia, J., and Zhang, M. (2007) "Clustering and synaptic targeting of PICK1 requires direct interaction between the PDZ domain and lipid membranes." EMBO J, 26, 4576-4587. (*: co-first authors)

2.Chen, J.*, Pan, L.*, Wei, Z., Zhao, Y., Zhang, M. (2008) "Domain Swapped Dimerization of ZO-1 PDZ2 Generates Specific and Regulatory Connexin43 Binding Sites" EMBO J, 27, 2113-2123. (*: co-first authors)

3.Pan, L.*, Yan, J.*, Wu, L., and Zhang, M.(2009) "Assembling Stable Hair-Cell Tip Link Complex via Multi-dentate Interactions between Harmonin and Cadherin23" PNAS. 1065575-5580. (*: co-first authors)

4.Wen, W., Yu, J., Pan, L., Wei, Z., Weng, J., Wang, W., Yan, S., Tran, T., Hong, W., and Zhang, M. (2009) "Lipid-induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6" Mol. Cell (in press)

5.Yan, J.*, Pan, L.*, Chen, X., Wu, L., and Zhang, M.(2009) "The Structure of the Harmonin/Sans Complex Reveals an Unexpected Interaction Mode of the Two Usher Syndrome Proteins" PNAS. (in press)(*: co-first authors)

6.Shi, Y., Yu, J., Jia, Y., Pan, L., Shen, C., Xia, J., and Zhang, M. (2009) " Redox-regulated lipid membrane binding of the PICK1 PDZ domain " (submitted)