报告题目：Metabolomics for Quantitative Assessment of Oxidative Stress by Capillary Electrophoresis-Mass Spectrometry
时间：2010年7月7日（星期三）上午9:30
地点：生命科学与技术学院二楼会议室
报告人：Professor Philip Britz-McKibbin
摘要：
Ageing and many human diseases are associated with oxidative stress, which is a condition reflected by a loss of redox signaling and control resulting in irreversible cellular damage by reactive oxygen/nitrogen species. Despite the wide use of biomarkers of oxidative damage in clinical research, accurate quantification of oxidative stress in vivo remains elusive. In this presentation, new strategies for artifact-free assessment of oxidative stress will be presented based on targeted and non-targeted metabolite profiling (i.e., metabolomics) of biological fluids using capillary electrophoresis-mass spectrometry (CE-MS). An introduction of CE/CE-MS technology and metabolomics will first be presented in the context of successful expanded newborn screening programs for early detection of inborn errors of metabolism [1, 2]. Recent strategies for artifact-free analysis of thiols will then be discussed when using CE-MS for determination of cellular redox status and antioxidant capacity based on the differential rates of glutathione oxidation [3]. A differential metabolomics study using CE-MS in conjunction with multivariate data analysis will also be presented in order to assess the efficacy of nutritional intervention to attenuate exercise-induced oxidative stress on a healthy volunteer [4]. The development of reliable analytical methodologies for metabolomics is critical towards improved understanding of the causal relationship between oxidative stress and human health.
References
[1] C. Kaiser, G. Seguí-Lines, J.C. D’Amaral and P. Britz-McKibbin Chem. Commun. 2008 338-340.
[2] K.R. Chalcraft and P. Britz-McKibbin Anal. Chem. 2009 81, 307-314.
[3] R. Lee and P. Britz-McKibbin Anal. Chem. 2009 81, 7047-7056.
[4] R. Lee, D. West, S.M. Phillips and P. Britz-McKibbin Anal. Chem. 2010 82, 2959-2968.
报告人简介：
Philip Britz-McKibbin
Department of Chemistry and Chemical Biology
McMaster University
1280 Main St. W.
Hamilton, ON L8S 4M1 Canada
Tel: +1-(905)-525-9140 ext.22771 E-mail: britz@mcmaster.ca
Webpage: http://www.chemistry.mcmaster.ca/britz/home.htm
EDUCATION
2000 University of British Columbia, Vancouver, BC, Canada
Ph.D. in Analytical Chemistry (PhD advisor: Dr. David. D.Y. Chen)
1994 University of Toronto, Toronto, ON, Canada
B.Sc. in Chemistry (Senior thesis advisor: Dr. Michael Thompson)
PROFESSIONAL EXPERIENCE
2009-current Associate Professor, Department of Chemistry and Chemical Biology, McMaster University, Canada
2003-2009 Assistant Professor, Department of Chemistry, McMaster University, Canada
2001-2003 Post-Doctoral Research Fellow, Graduate School of Material Sciences
Himeji Institute of Technology, Kamigori, Japan (PDF advisor: Dr. Shigeru Terabe)
2000-2001 Visiting Lecturer, Department of Chemistry and Biochemistry
University of North Carolina at Greensboro, Greensboro, NC, USA
HONORS AND AWARDS
2010 ACS Division of Analytical Chemistry Award for Young Investigators in Separation Science
2009 Japan Society for Promotion of Science Invited Fellowship (Long-term)
2007 Petro-Canada Young Investigator Award, McMaster University
2003/4/6Journal of Chromatography A: Top Referee Award, Elsevier
2004 Premier’s Research Excellence Award, Province of Ontario
2004 Centre for Leadership and Learning Grant Award, McMaster University
2001 Japan Society for Promotion of Science (JSPS) Postdoctoral Fellowship
EDITORIAL BOARD
2006-present Amino Acids, Section Editor, Springer
MEMBERSHIP
Canadian Chemical Society, American Chemical Society, Metabolomics Society
CV_2010 Britz-McKibbin, Philip 12b
Research Interests
Bio-analytical chemistry, Separation science, CE-MS, Metabolomics, Metabolite profiling, Oxidative stress, Protein unfolding, Biomolecular interactions, Enzyme kinetics, Boronic acids, Clinical chemistry
Publications List (Selected)
Assistant/Associate Professor Contributions (McMaster University)
i) Published Refereed Papers (Peer-reviewed; Selected)
1. F. Fei‡ and P. Britz-McKibbin* “Direct Analysis of Polyols using 3-Nitrophenylboronic Acid in Capillary Electrophoresis: Thermodynamic and Electrokinetic Principles of Molecular Recognition” Anal. Bioanal. Chem. 2010 accepted.
2. R. Lee† and P. Britz-McKibbin* “Metabolomic Studies of Radiation-induced Apoptosis of Human Leukocytes by Capillary Electrophoresis-Mass Spectrometry and Flow Cytometry: Adaptive Cellular Responses to Ionizing Radiation” Electrophoresis 2010 in press.
3. P. Britz-McKibbin* “Capillary Electrophoresis-Mass Spectrometry in Metabolomics” In: Thomas O. Metz (Editor) Methods in Molecular Biology: Metabolic Profiling, Humana Press 2010 in press.
4. J.M.A. Gavina,† C.E. White†, T.M. Finanº and P. Britz-McKibbin* “Determination of 4-Hydroxyproline-2-Epimerase Activity by Capillary Electrophoresis” A Stereoselective Platform for Inhibitor Screening of Amino Acid Isomerases” Electrophoresis 2010 in press.
5. R. Lee,† D. West,† S.M. Phillipsº and P. Britz-McKibbin* “Differential Metabolomics for Quantitative Assessment of Oxidative Stress with Strenuous Exercise and Nutritional Intervention: Thiol-specific Regulation of Cellular Metabolism by N-Acetyl-L-Cysteine” Anal. Chem. 2010 82, 2959-2968.
6. R. Lee† and P. Britz-McKibbin* “Differential Rates of Glutathione Oxidation for Assessment of Cellular Redox Status and Antioxidant Capacity by Capillary Electrophoresis-Mass Spectrometry: An Elusive Biomarker of Oxidative Stress” Anal. Chem. 2009 81, 7047-7056.
7. K.R. Chalcraft,† R. Lee,† C. Mills‡ and P. Britz-McKibbin* “Virtual Quantification of Metabolites by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry: Predicting Ionization Efficiency without Chemical Standards” Anal. Chem. 2009 81, 2506-2515.
8. J.M.A. Gavina,† M.T. Mazhab-Jafari†, G. Melaciniº and P. Britz-McKibbin* “Label-free Assay for Thermodynamic Analysis of Protein-ligand Interactions: A Multivariate Strategy for Allosteric Ligand Screening” Biochemistry 2009 48, 223-225 (Rapid Reports). #2 Most Assessed Rapid Reports Article in Biochemistry for 2009.
9. K.R. Chalcraft† and P. Britz-McKibbin* “Newborn Screening of Inborn Errors of Metabolism by Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry: A Second-Tier Method with Improved Specificity and Sensitivity” Anal. Chem. 2009 81, 307-314.
10. A.S. Ptolemy† and P. Britz-McKibbin* “New Advances in On-line Sample Preconcentration by Capillary Electrophoresis using Dynamic pH Junction” Analyst, 2008, 133, 1643-1648 (Highlight).
11. C. Kaiser,‡ G. Seguí-Lines,† J.C. D’Amaral‡ and P. Britz-McKibbin* “Electrokinetic Probes for Single-step Screening of Polyol Stereoisomers: The Virtues of Ternary Boronate Ester Complexation” Chem. Commun. 2008 338-340.
12. G. Seguí-Lines,† J.M.A. Gavina,† J.C. D’Amaral‡ and P. Britz-McKibbin* "High-throughput Screening of Holoprotein Conformational Stability by Dynamic Ligand Exchange-Affinity Capillary Electrophoresis” Analyst 2007 132, 741-744 (Communication).
13. A.S. Ptolemy,† R. Lee† and P. Britz-McKibbin* "Strategies for Comprehensive Analysis of Amino Acids as Biomarkers of Oxidative Stress” Amino Acids 2007 33, 3-18 (Invited Review).
15. R. Lee,† A. S. Ptolemy,† L. Niewzcas‡ and P. Britz-McKibbin* “Integrative Metabolomics for Characterizing Unknown Low Abundance Metabolites by Capillary Electrophoresis-Mass Spectrometry with Computer Simulations” Anal. Chem. 2007 79, 403-415 (Accelerated Article). #9 Most Assessed Article in Analytical Chemistry for Winter 2007.
